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KMID : 1160619990040030171
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Preventive Nutrition and Food Science
1999 Volume.4 No. 3 p.171 ~ p.174
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High Performance Liquid Chromatography (HPLC) Detection of Malonaldehydethiobarbituric Acid (MA-TBA) Complex in Ground Pork
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Whang Key
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Abstract
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For monitoring lipid oxidation development in cooked ground pork during refrigerationm, malonaldehydethiobarbituric acid(MA-TBA) contents were measured using high performance liquid chromatography(HPLC). As the oxidation proceeded during refergeration, TBA-reaction substances(TBARS) absorbances increased and the corresponding HPLC peak areas also increased proportationately. The correlation coefficient between the HPLC peak areas and MA-TBA absorbance were 0.9979. The treatemtn of cetrimide, an ion pairing agent, gave a complete resolution of the MA-TBA complex and the butanol extraction of the complex increased its recovery by 37.8%. Both cetrimide treatment and butanol extraction are essential steps for analyzing MA-TBA complex in ground pork wiht HPLC. A reliable and specific measurement of NA-TBA in ground pork was successfully performed using HPLC.
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KEYWORD
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MA-TBA content, ground pork, high performance liquid chromatography, ion-paring agent, gutanol extraction
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