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KMID : 1160619990040030209
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Preventive Nutrition and Food Science
1999 Volume.4 No. 3 p.209 ~ p.214
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Ethanol-induced Activiationof Transcription Factor NF-κB and AP-1 in C6 Glial Cells
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Park Jae-Won
Shim Young-Sup
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Abstract
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In this study, the effects of ethanol and acetaldehyde on DNA binding activities of NF-κB and AP-1 were evaluated in C6 rat glial cells. Both NF-κB and AP-1 are important transcription factors for the expression of various cytokines in glial cells. Our data showed that neither ethanol nor acetaldehyde induced conspicuous cell death of C6 cells at clinically realistic concentrations. When the DNA binding activities of nuclear NF-κB and AP-1 were estimated using electrophoretic mobility shift assay (EMSA), ethanol (0.3%) or acetaldehyde (1 mM) induced transient activation of these transcription factors, which attained peak levels at 4~8 hours and declined to basal levels at 12 hours after treatment. The supershift analysis showed that the increased activities of NF-κB in ethanol/acetaldehyde-treated C6 cells were due to the preferential induction of p65/p50 heterodimer complex. The DNA binding activities of these transcriptional factors decreased below basal levels when cells were cultured with either ethanol or acetaldehyde for 24 hours, and showed the inhibitory effects of chronic ethanol/acetaldehyde treatment on the activities of these transcriptional factors. Our data indicate that either ethanol or acetaldehyde can induce functional changes of glial cells through bi-directional modulation of NF-κB and AP-1 DNA binding activities.
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KEYWORD
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glia l, ethanol, acetaldehyde, NF-κB, AP-1
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