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KMID : 1160620000050010010
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Preventive Nutrition and Food Science
2000 Volume.5 No. 1 p.10 ~ p.14
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A Simple Method for the Preparation of Highly Pure Conjugated Linoleic Acid(CLA) Synthesized from Safflower Seed Oil
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Kim Young-R.
Lee Young-H.
Park Kyung-A.
Ha Yeong-L.
Kim Jeong-O.
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Abstract
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A simple and rapid method was developed to prepare a large quantity of highly pure conjugated linoleic acid (CLA) chemically-synthesized from safflower seed oil (SSO). CLA-SSO (74.9% in purity) was synthesized from fresh SSO (79.9% of linoleic acid) by alkaline isomerization at 180℃. Urea (50 g) and CLA-SSO (25 g) were completely dissolved in ethanol (750 ml) using a water bath (50℃) and followed by refluxing for 60 min. The resultant was cooled to room temperature and stored in a cold room (4℃) for 24 hrs. After removing the urea adduct by filtration, the filtrate was rotoevaporated under 40℃ and the residue was dissolved in hexane (200 ml). The hexane extract was washed with distilled water (100 ml×3) and dried over sodium sulfate anhydrous. This urea treatment procedure was repeated three times. The purity of CLA recovered from the hexane extract was 95.0%. This method can be applied to prepare a large quantity of highly pure chemically-synthesized CLA (>0.5kg/a batch) from any plant oils containing high percentages (>70%) of linoleic acid.
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KEYWORD
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conjugated linoleic acid(CLA), urea adduct
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