|
KMID : 1160620000050040179
|
|
Preventive Nutrition and Food Science
2000 Volume.5 No. 4 p.179 ~ p.183
|
|
|
The Preparation and Identification of Hydrolysis Oligosaccharide from White Copra Meal by Yeast Fermentation and Sunflower Seed Enzymes
|
|
Park Gwi-Gun
|
|
|
Abstract
|
|
|
|
β-1,4-Mannotriose was prepared by the enzymatic hydrolysis of white copra meal (WCM) and the subsequent elimination of monosaccharides from the resultant hydrolysate with a yeast. The enzyme system from sunflower seed hydrolyzed WCM and produced monosaccharides and β-1,4-mannotriose without other oligomers at the final stage of the reaction. WCM (50 g) was hydrolyzed at 50℃ and pH 4.5 for 24 hr with a crude enzyme solution (500 mL) from sunflower seed. By the elimination of monosaccharides from the hydrolysis products with a yeast (Candida glaebosa), 8.1 g of crystalline mannotriose was otained without the use of chromatographic techniques. After 48hr of yeast cultivation, the total sugar content decreased from 4.6% to 3.5%, whereas the average degree of polymerization increased from 2.3 to 3.1.
|
|
|
KEYWORD
|
|
|
β-1,4-mannotriose, white copra meal, sunflower seed, α-galactosidase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|