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KMID : 1160620010060020091
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Preventive Nutrition and Food Science
2001 Volume.6 No. 2 p.91 ~ p.95
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Immunological Assay to Detect Irradiated Beef
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Lee Ju-Woon
Yook Hong-Sun
Lee Hyun-Ja
Kim Jung-Ok
Byun Myung-Woo
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Abstract
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Competitive indirect enzyme linked immunosorbent assay (Ci-ELISA) was used to obtain the preliminary data for the detection of irradiated beef. Ci-ELISA was individually formatted with polyclonal antibodies produced from 2 kinds of bovine proteins, myosin and bovine serum albumin (BSA). Beef round, loin and tender loin were vacuum-packaged and subdivided into 3 groups of 1) irradiation; 2) irradiation and chilled at 4℃ for 7 day; 3) irradiation and frozen at 20℃ for 2 months to observe the changes under different storage and/or distribution conditions. Irradiation was performed at 3, 5 and 7 kGy. Protein solutions prepared from the sample were tested by formatted Ci-ELISA. Detected concentrations of myosin and BSA decreased with the increased irradiation dose in all samples with different reduction rates. Myosin was more susceptible to freezing than BSA. Samples irradiated at 5 kGy or above could be differentiated from non-irradiated ones by Ci-ELISA. These results indicate that immunological assay can be used as a detection method for irradiated beef.
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KEYWORD
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Ci-ELISA, myosin, BSA, irradiated beef, detection method
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