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KMID : 1160620010060040206
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Preventive Nutrition and Food Science
2001 Volume.6 No. 4 p.206 ~ p.210
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Ethanol Fermentation of Corn Starch by a Recombinant Saccharomyces cerevisiae Having Glucoamylase and α-Amylase Activities
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Lee Dae-Hee
Park Jong-Soo
Ha Jung-Uk
Lee Seung-Cheol
Hwang Yong-Il
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Abstract
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Starch is an abundant resource in plant biomass, and it should be hydrolyzed enzymatically into fermentable sugars for ethanol fermentation. A genetic recombinant yeast, Saccharomyces cerevisiae GA-7458, was constructed by integrating the structural gene of both α-amylase from Bacillus stearothermophilus and the gene (STA1) encoding glucoamylase from S. diastaticus into the chromosome of S. cerevisiae SH7458. The recombinant yeast showed active enzymatic activities of α-amylase and glucoamylase. The productivity of ethanol fermentation from the pH-controlled batch culture (pH 5.5) was 2.6 times greater than that of the pH-uncontrolled batch culture. Moreover, in a fed-batch culture, more ethanol was produced (13.2 g/L), and the production yield was 0.38 with 2% of corn starch. Importantly, the integrated plasmids were fully maintained during ethanol fermentation.
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KEYWORD
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ethanol fermentation, glucoamylase, α-amylase, fed-batch culture
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