|
KMID : 1160620020070040353
|
|
Preventive Nutrition and Food Science
2002 Volume.7 No. 4 p.353 ~ p.357
|
|
|
Water Extract of Kudzu Root (Pueraria radix) Decreases Apolipoprotein B100 and B48 Production in Vitro
|
|
Lee Jeong-Sook
|
|
|
Abstract
|
|
|
|
We have previously demonstrated that kudzu root extracts have a hypocholesterolemic effect on rats fed diets high in fat and cholesterol. To further elucidate the mechanism involved, in this study we investigated the effect of water extracts of kudzu root, Pueraria radix, on the production of apolipoprotein B100 (Apo B100) in HepG2 liver cells and secretion of apolipoprotein B48 (Apo B48) in Caco2 cells. Human cell lines, HepG2 liver cells and Caco2 intestinal epithelial cells, were grown with various concentrations (0%, 0.5%, 1.0%, 1.5%, 2.0%) of water extracts of kudzu root in the media. The kudzu root extract decreased Apo B100 production and secretion. Treatment of Hep G2 cells with the kudzu root extract also significantly decreased the intracellular total and free cholesterol concentration, and also decreased esterified cholesterol but was only significant at the highest dose of 2%. Apo B48 production, but not secretion, from enterocytes was lowered by the kudzu root extracts. This research provided evidence that the hypocholesterolemic properties of kudzu root may be a consequence of decreased production and secretion of Apo B100 in the liver and Apo B48 in the intestine.
|
|
|
KEYWORD
|
|
|
Pueraria radix, kudzu, Apo B100, APo G2 cells, Caco2 cells, cholesterol
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|