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KMID : 1160620020070040421
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Preventive Nutrition and Food Science
2002 Volume.7 No. 4 p.421 ~ p.426
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Purification and Characterization of an Intracellular Inulinase from Bacillus sphaericus 188-1
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Kim Jae-Ho
Kwak Yoon-Jin
Lee Jong-Tae
Park Shin-Yang
Lee Jong-Soo
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Abstract
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In order to obtain basal data for industrial application of inulinase from Bacillus sphaeicus 188-1, its intracellular inulinase was purified by ammonium sulfate fractionation and column chromatography on DEAE-Sephadex A-50 and Sephadex G-100. The enzyme was homogeneous as judged by SDS-polyacrylamide gel electrophoresis, with an apparent molecular weight of 29 kDa. Inulinase activity was optimal at pH 6.5 and 40oC. The enzyme activity was significantly inhibited by Cu2+, Cd2+ and Hg2+. The inulinase exhibited an apparent Km value of 0.014% for inulin.
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KEYWORD
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Bacillus sphaericus 188-1, intracellular inulinase, characterization
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