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KMID : 1160620030080010066
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Preventive Nutrition and Food Science
2003 Volume.8 No. 1 p.66 ~ p.69
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Purification of an ACE Inhibitory Peptide from Hydrolysates of Duck Meat Protein
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Kim So-Youn
Kim Sun-Hye
Song Kyung-Bin
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Abstract
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An angiotensin converting enzyme (ACE) inhibitory peptide was isolated and purified from the hydrolysates of duck meat protein. Duck meat protein was hydrolyzed using trypsin at 37℃ for 2 hrs. An ACE inhibitory peptide was purified using membrane filtration, anion exchange chromatography, gel permeation chromatography, fast protein liquid chromatography, normal phase HPLC. The purified inhibitory peptide was identified to be a tetrapeptide, Glu-Asp-Leu-Glu having IC50/ value of 85.9 ㎛.
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KEYWORD
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ACE inhibitor, duck protein, hydrolysates, isolation
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