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KMID : 1160620030080020124
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Preventive Nutrition and Food Science
2003 Volume.8 No. 2 p.124 ~ p.129
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Suppressive Effect of Chlorella Methanol Extract on Oxidative Stress and NFkB Activation in RAW 264.7 Macrophages
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Park Ji-Young
Lee Hyo-Sun
Song Young-Sun
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Abstract
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This study was designed to investigate whether a methanol extract of chlorella can suppress oxidative stress and nuclear factor kB (NFkB) activation in lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophage cells. Treatment of RAW 264.7 cells with chlorella methanol extract (25, 50, and 100 ㎍/mL) significantly reduced LPS-stimulated nitric oxide production in a dose-dependent manner. Treatments with chlorella methanol extract at all concentrations also reduced thiobarbituric acid-reactive substances accumulation and enhanced glutathione level at 50 and 100 ㎍/mL levels. The specific DNA binding activities of NFkB on nuclear extracts in cells treated with 50 ㎍/mL and 100 ㎍/mL chlorella methanol extracts were significantly suppressed. These results suggest that chlorella methanol extract has mild antioxidative activity and the ability to suppress intracellular oxidative stress and NFkB activation.
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KEYWORD
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chlorella methanol extract, oxidative stress, NFkB, NO, macrophages
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