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KMID : 1160620030080020130
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Preventive Nutrition and Food Science
2003 Volume.8 No. 2 p.130 ~ p.136
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Effect of Onion Extract on the Carbon Tetrachloride-induced Liver Injury in Mouse
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Lee Kyung-Jin
Kim Deok-Song
Kim Jong-Sun
Chin Jong-Eun
Kim Jun-Ho
Na Myung-Suk
Lee Jong-Bin
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Abstract
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The protective effects of onion extract (OE), onion powder extracted in ethanol for 2 days. on carbon tetrachloride (CCl4)-induced hepatotoxicities and the possible mechanisms involved in this protection were investigated in mice. Pretreatment with OE prior to the administration of CCl4 significantly reduced the increase in serum alanine and aspartate aminotransferase activities and hepatic lipid peroxidation in a dose-dependent manner. In addition, pretreatment with OE significantly prevented the depletion of reduced glutathione content in the liver of CCl4-intoxicated mice. CCl4-induced hepatotoxicity was also prevented, as indicated by a liver histopathologic findings. The effects of OE on the cytochrome P450 (P450) 2E1, the major isozyme involved in CCl4 biotransformation were investigated. Treatment of mice with OE resulted in a significant decrease in P450 2E1-dependent p-nitrophenol and aniline hydroxylation in a dose-dependent manner. Consistent with these observations, the P450 2E1 expressions were also decreased, as determined by immunoblot analysis. OE also exhibited antioxidant effects in FeCl2-ascorbate induced lipid peroxidation in rat liver homogenates and in superoxide radical scavenging activity. These results show that the protective effects of OE against the CCl4-induced hepatotoxicity may be due to its ability to block bioactivation of CCl4, mainly tty inhibiting the expression and activities of P450 2E1 and by scavenging free radicals.
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KEYWORD
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onion extract (OE), carbon tetrachloride (CCl4), cytochrome P450 2E1, antioxidant
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