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KMID : 1160620030080040301
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Preventive Nutrition and Food Science
2003 Volume.8 No. 4 p.301 ~ p.305
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Determination of Vitamin B12 (Cyanocobalamin) in Fortified Foods by HPLC
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Park Youn-Ju
Jang Jae-Hee
Park Hye-Kyung
Koo Yong-Eui
Hwang In-Kyeong
Kim Dai-Byung
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Abstract
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This study was conducted to develop an HPLC method for determining vitamin B12 in fortified foods which has typically been determined by microbiological assays according to AOAC and Korean Food Code approved methods. Vitamin B12 (cyanocobalamin) was determined by reversed-phase HPLC with a triple column and UV/VIS dectector (550 nm) using the column switching technique after extraction with 5 mM potassium phosphate solution by sonication without a clean-up procedure. The recovery of spiked samples and limit of detection (LOD) by HPLC were 78.6 ∼107.5 % and 2 ppb (㎍/kg), respectively. The LOD of the microbiological assay (MBA) was much lower than that of HPLC. The concentrations of vitamin B12 analyzed in all tested samples (n=12) confirmed compliance with declared label claims. The range of recovery ratio by the HPLC method when compared to the microbiological assay was 76.2 ∼140.0 %. There was not significant difference between the HPLC and MBA methods (p < 0.01) with r=0.9791 and linear regression y=0.9923x-0.04. The HPLC method for determining vitamin B12 using the column-switching technique appears to be suitable for determining vitamin B12 concentrations above 1 ㎍/100 g in fortified foods.ied foods.
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KEYWORD
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vitamin B12, HPLC method, microbiological assay
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