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KMID : 1160620030080040356
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Preventive Nutrition and Food Science
2003 Volume.8 No. 4 p.356 ~ p.364
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Differential Effects of Resveratrol and its Oligomers Isolated from Seeds of Paeonia lactiflora (Peony) on Proliferation of MCF-7 and ROS 17/2.8 Cells
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Kim Hyo-Jin
Lee Won-Jung
Park Yun-Hee
Cho Sung-Hee
Park Sang-Won
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Abstract
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A methanol extract from seeds of Paeonia lactiflora (Paeoniaceae, peony) was found to possess different antiproliferative activities against four different human cancer cell lines: Hela, MCF-7, HepG2 and HT-29. Furthermore, five different methanol (20, 40, 60, 80 and 100% MeOH) fractions obtained by fractionation of the methanol extract of the seeds on a Diaion HP-20 column exhibited differential antiproliferative effects against the above four cancer cell lines. Among five fractions, the 60% MeOH fraction showed relatively lower antiproliferative activity on MCF-7 estrogen-sensitive breast cancer cell than the other cancer cell lines. Systematic separation of 60% the MeOH fraction by silica gel and Sephadex LH-20 columns led to the isolation of four known stilbenes, trans-resveratrol (1), trans-(+)- ε-viniferin (2), gnetin H (3) and suffruticosol B (4). The four stilbenes (1～4) exerted differential biphasic effects on cell proliferation of MCF-7 cells in a similar manner as genistein, a soybean isoflavone used as a positive reference, in the concentration range from 1.0 to 200 μM. Three stilbenes (1～3) weakly stimulated the proliferation of MCF-7 cells at doses below 10 μM. However, strong antiproliferative effects on MCF-7 cell were exerted by extract 1 at a dose of 200 μM, and by 2 and 3 at doses above 25 μM. In contrast, 4 inhibited the proliferation of MCF- 7 cell at a dose below 25 μM, but stimulated cell proliferation at concentrations of 50 and 100 μM. All four stilbenes (1～4) stimulated the proliferation of ROS 17/2.8 osteoblast-like cells in the range of 10^(-10)～10^(-1) μM. Compound 1 exhibited especially potent proliferative activity, although its activity was weaker than that of genistein. Additionally, three resveratrol oligomers (2～4) also exhibited concentration-dependently moderate proliferative activity, but less than that of 1. These results suggest that resveratrol, and its dimer and trimers from the seeds of Paeonia lactiflora may act as a phytoestrogen, but in a somewhat different manner from that of genistein.
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KEYWORD
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phytoestrogen, Paeonia lactiflora, Paeoniaceae, stilbenes, trans-resveratrol, trans-(+)-
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