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KMID : 1160620040090020187
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Preventive Nutrition and Food Science
2004 Volume.9 No. 2 p.187 ~ p.189
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Isolation of a Tryptophan-Overproducing Strain Generated by EMS Mutagenesis of Candida rugosa
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Kim Sun-Hye
Ha Yu-Mi
Youm Hyoung-Joon
An Gil-Hwan
Lee Bong-Duk
Won Mi-Sun
Song Kyung-Bin
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Abstract
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To isolate a mutant strain which overproduces tryptophan, mutants of Candida rugosa were screened after EMS (ethyl methane sulfonate) mutagenesis. Fluorotryptophan, a tryptophan analogue, was used for selection of a tryptophan-overproducing mutant after mutagenesis. Among 50 mutants, several candidates were selected based on intracellular tryptophan content. Amino acid analysis results showed that C3 was the best strain because it had the highest amount of tryptophan among the mutants.
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KEYWORD
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amino acid analog, Candida rugosa, tryptophan, EMS mutagenesis
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