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KMID : 1160620040090040295
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Preventive Nutrition and Food Science
2004 Volume.9 No. 4 p.295 ~ p.299
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Characterization of Thermal Products of Alpha-Tocopherol
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Chung Hee-Young
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Abstract
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Alpha-tocopherol was thermally oxidized and degraded at high temperatures and the resulting products were chromatographically separated and identified by LC-MS. Alpha-tocopherol dissolved in glycerol was heated at 200℃ for 30 min. The thermal products were separated by hexane extraction and analyzed by HPLC using a reversed phase μ-Bondapak C_(18)-column with two kinds of elution solvents: a mixture of acetonitrile and methanol (3:2), and of acetonitrile, methanol, 2-propanol, chloroform and methylene chloride (3:2:5:0.5:0.5) in a gradient mode. The isolated thermal products of alpha-tocopherol were more viscous than alpha-tocopherol, and dark brown in color. Major thermal degradation products of alpha-tocopherol were identified by LC-MS, and the structures of thermal products were proposed. Alpha-tocopherol and its thermal degradation products were degraded into fragments, mainly at the non-aromatic parts. The degradation products of alpha-tocopherol were combined with oxidized product (tocopherylquinone) to make thermal products through dimerization.
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KEYWORD
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alpha-tocopherol, thermal degradation products, HPLC
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