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KMID : 1160620050100040316
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Preventive Nutrition and Food Science
2005 Volume.10 No. 4 p.316 ~ p.325
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Characterization of Antioxidant Potential of a Methanolic Extract and Its Fractions of Highbush Blueberry (Vaccinium corymbosum L.)
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Senevirathne Mahinda
Jeon You-Jin
Ha Jin-Hwan
Lee Chi-Ho
Cho So-Mi K.
Kim Soo-Hyun
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Abstract
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The antioxidant potential of a 75% methanolic extract of highbush blueberry (Vaccinium corymbosum L.) and its different fractions was investigated using different reactive oxygen species (ROS), nitric oxide (NO·), metal chelating and lipid peroxidation assays. Methylene chloride and 75% methanol fractions showed equally high activities (IC_(50) 0.010 mg/mL) for hydroxyl radical (HO·) scavenging. Higher hydrogen peroxide (H₂O₂) scavenging values were reported for the ethyl acetate and methylene chloride fractions and their IC_(50) values were 0.20 and 0.15 mg/mL, respectively. Nitric oxide (NO·) and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging activities were higher in ethyl acetate and methylene chloride fractions. Chloroform and water fractions showed higher activities in superoxide (O₂·) scavenging. All fractions showed strong metal chelating capacities compared with the commercial antioxidants tested. The 0.1% ethyl acetate fraction showed notable capacity to suppress lipid peroxidation in both fish oil and linoleic acid. Phenolic content was measured in all the fractions and methanolic extract. Among the fractions, ethyl acetate fraction showed the highest phenolic content.
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KEYWORD
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highbush blueberry (Vaccinium corymbosum L.), antioxidant potential, reactive oxygen species scavenging, methanolic extract, total phenolics
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