|
KMID : 1160620080130040259
|
|
Preventive Nutrition and Food Science
2008 Volume.13 No. 4 p.259 ~ p.265
|
|
|
Fucoidan Protects LLC-PK1 Cells against AAPH-induced Damage
|
|
Park Min-Jung
Han Ji-Sook
|
|
|
Abstract
|
|
|
|
This study was designed to investigate the protective effect of fucoidan against AAPH-induced oxidative stress in LLC-PK1 cells (porcine kidney epithelial cells). Oxidative stress was induced by exposing of LLC-PK1 cells to the 1 mM 2,2'-azobis(2-amidino propane) dihydrochloride (AAPH) for 24 hr. Exposure of LLC-PK1 cells to 1 mM AAPH for 24 hr resulted in a significant (p<0.05) decrease in cell viability, but fucoidan treatment protected LLC-PK1 cells from AAPH-induced cell damage in a dose dependant manner. To investigate the protective action of fucoidan against AAPH-induced damage of LLC-PK1 cells, we measured the effects of fucoidan on lipid peroxidation and antioxidant enzymes activities of AAPH treated cells as well as scavenging activities on superoxide anion radical and hydroxyl radical. Fucoidan had protective effect against the AAPH-induced LLC-PK1 cellular damage and decreased lipid peroxidation and increased activities of antioxidant enzymes such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-px). Furthermore, fucoidan showed strong scavenging activity against superoxide anion radical. The IC50 value of fucoidan was 48.37±1.54 μg/mL for superoxide anion radical scavenging activity. The fucoidan also had high hydroxyl radical scavenging activity (IC50=32.03 μg/mL). These results indicate that fucoidan protects against AAPH-induced LLC-PK1 cell damage by inhibiting lipid peroxidation, increasing antioxidant enzyme activities and scavenging offree radicals.
|
|
|
KEYWORD
|
|
|
fucoidan, AAPH, radical scavenging, antioxidant activity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|