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KMID : 1160620090140030260
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Preventive Nutrition and Food Science
2009 Volume.14 No. 3 p.260 ~ p.264
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Determination of Tocopherol Contents in Refined Edible Oils Using an HPLC Method
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Hu Jiang-Ning
Zhu Xue-Mei
Adhikari Prakash
Li Dan
Kim In-Hwan
Lee Ki-Teak
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Abstract
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A high-performance liquid chromatography method was applied to determine the contents of tocopherols in edible oils using a LiChrosorb DIOL HPLC column and hexane fortified with 0.1% acetic acid in an isocratic mode. The validation of the method included tests for linearity, sensitivity, accuracy, precision, and recovery. All calibration curves showed good linear regression (r2>0.9995) within the tested ranges. The established method offered good precision and accuracy with overall intra-day and inter-day variations of 0.94～4.27 and 1.77～4.88%, respectively. The tocopherol recoveries ranged from 91.44～108.90%. Subsequently, the method was successfully applied to qualitatively and quantitatively determine the total contents of α, γ, and δ-tocopherols in 12 selected refined edible oils, showing a range of 0.92 to 188.71 mg/100 g.
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KEYWORD
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refined edible oils, tocopherols, HPLC separation
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