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KMID : 1160620100150030206
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Preventive Nutrition and Food Science
2010 Volume.15 No. 3 p.206 ~ p.212
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Codium fragile Ethanol Extraction Inhibited Inflammatory Response through the Inhibition of JNK Phosphorylation
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Han Sin-Hee
Kim Young-Guk
Lee Su-Hwan
Park Chung-Berm
Choi Han-Gil
Jang Hye-Jin
Lee Young-Seob
Kwon Dong-Yeul
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Abstract
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Codium fragile (CF) is an edible green alga consumed as a traditional food source in Korea. In this study, the?ethanol extract of CF was evaluated to determine if it has anti-inflammatory activity. Lipopolysaccharide (LPS), a toxin from bacteria, is a potent inducer of inflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-6. Therefore, we studied whether CF extracts have an anti-inflammatory effect in LPS-induced murine macrophage cell lines (RAW 264.7). In the present study, IL-6 production was measured using an enzyme- linked immunosorbent assay (ELISA), prostaglandin E2 (PGE2) production was measured using the EIA kit, and cyclooxygenase (COX)-2 and mitogen-activated protein kinase (MAPK) activation were determined by Western blot analysis. IL-6 mRNA, COX-2 mRNA and iNOS mRNA expression were measured using reverse transcription- polymerase chain reaction (RT-PCR). The results indicated that CF extracts inhibit LPS-induced IL-6, NO and PGE2 production in a dose-dependent manner, as well as expression of iNOS and COX-2. CF extracts significantly inhibited LPS-induced c-Jun N-terminal kinase (JNK) 1/2 phosphorylation. Taken together, these findings may help elucidate the mechanism by which CF modulates RAW 264.7 cell activation under inflammatory conditions.
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KEYWORD
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Codium fragile, lipopolysaccharide, anti-inflammatory effect
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