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KMID : 1160620110160010029
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Preventive Nutrition and Food Science
2011 Volume.16 No. 1 p.29 ~ p.36
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Determination of Phytochemical Contents and Biological Activities from the Fruits of Elaeagnus multiflora
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Lee Jin-Hwan
Seo Weon-Taek
Cho Kye-Man
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Abstract
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The purpose of this study was to investigate the phytochemical contents and biological activities, including α-glucosidase inhibitory activity and antioxidant activity, from the fruits of Elaeagnus multiflora using different solvent systems. The 50% ethanol extract of this species showed the most potent α-glucosidase inibitory activity (88.5%) at a concentration of 1 mg/mL. Moreover, this extract also displayed the strongest antioxidant activity in each assay, showing 96.3% in DPPH scavenging activity, 98.2% in ABTS scavenging activity, and 2.5% in reducing power. The highest total phytochemical contents, including flavan-3-ols, phenolic acids, and flavonoids, were observed in the 50% ethanol extract. Interestingly, flavan-3-ols (3.150 mg/g) were detected at a significantly higher total content than those of phenolic acids (0.380 mg/g) and flavonoids (not detected). Additionally, the contents of individual phytochemicals showed remarkable differences, especially the epicatechin gallate (2.008 mg/g) and gallic acid (0.099 mg/g), which were the predominant constituents of each phytochemical type. Therefore, our results suggest that the 50% ethanol extract of E. multiflora fruits has strong biological activities, which are correlated with high phytochemical contents.
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KEYWORD
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Elaeagnus multiflora, fruits, phytochemical, antioxidant, α-glucosidase, 50% ethanol extract
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