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KMID : 1160620110160020174
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Preventive Nutrition and Food Science
2011 Volume.16 No. 2 p.174 ~ p.178
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Effect of Particle Size of Zinc Oxides on Cytotoxicity and Cell Permeability in Caco-2 Cells
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Chang Hyun-Joo
Choi Sung-Wook
Ko Sang-Hoon
Chun Hyang-Sook
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Abstract
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The cell permeability and cytotoxic effects of different-sized zinc oxide (ZnO) particles were investigated using a human colorectal adenocarcinoma cell line called Caco-2. Morphological observation by scanning electron microscopy revealed that three zinc oxides with different mean particle sizes (ZnO-1, 20 nm; ZnO-2, 90～200 nm; ZnO-3, 1～5 μm) tended to aggregate, particularly in the case of ZnO-1. When cytotoxicities of all three sizes of zinc oxide particles were measured at concentration ranges of 1～1000 μg/mL, significant decreases in cell viability were observed at concentrations of 50 μg/mL and higher. Among the three zinc oxides, ZnO-1 showed the lowest viability at 50 μg/mL in Caco-2 cells, followed by ZnO-2 and ZnO-3. The permeate concentration of ZnO-1 from the apical to the basolateral side in the Caco-2 model system after four hours was about three-fold higher than that of either ZnO-2 or ZnO-3. These results demonstrated that ZnO-1, with a 20 nm mean particle size, had poorer viability and better permeability in Caco-2 cells than ZnO-2 and ZnO-3.
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KEYWORD
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zinc oxide, Caco-2, cytotoxicity, cell permeability, particle size effect
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