|
KMID : 1160620120170020116
|
|
Preventive Nutrition and Food Science
2012 Volume.17 No. 2 p.116 ~ p.121
|
|
|
Antioxidant Activity and Protection from DNA Damage by Water Extract from Pine (Pinus densiflora) Bark
|
|
Jiang Yunyao
Han Woong
Shen Ting
Wang Myeong-Hyeon
|
|
|
Abstract
|
|
|
|
Water extract from Pinus densiflora (WPD) was investigated for its antioxidant activity and its ability to provide protection from DNA damage. A series of antioxidant assays, including a 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging assay, a reducing power assay, a metal-chelating assay, a superoxide radical scavenging assay, and a nitrite scavenging ability, as well as a DNA damage protection assay were performed. Total phenolic content was found to be 211.32 mg Tan/g WPD. The extract scavenged 50% DPPH free radical at a concentration of 21.35 μg/mL. At that same concentration, the reducing power ability of WPD was higher than that of α -tocopherol. The extract chelated 68.9% ferrous ion at the concentration of 4 mg/mL. WPD showed better nitrite scavenging effect at the lower pH. Meanwhile, WPD exhibited a strong capability for DNA damage protection at 1 mg/mL concentration. Taken together, these data suggest water extract from Pinus densiflora could be used as a suitable natural antioxidant.
|
|
|
KEYWORD
|
|
|
antioxidant, DNA damage, phenolic content, reactive oxygen species
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|