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KMID : 1160620130180020124
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Preventive Nutrition and Food Science
2013 Volume.18 No. 2 p.124 ~ p.132
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Hepatic Fibrosis Inhibitory Effect of Peptides Isolated from Navicula incerta on TGF-b1 Induced Activation of LX-2 Human Hepatic Stellate Cells
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Kang Kyong-Hwa
Qian Zhong-Ji
Ryu Bo-Mi
Karadeniz Fatih
Kim Dae-kyung
Kim Se-Kwon
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Abstract
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In this study, novel peptides (NIPP-1, NIPP-2) derived from Navicula incerta (microalgae) protein hydrolysate were explored for their inhibitory effects on collagen release in hepatic fibrosis with the investigation of its underlying mechanism of action. TGF-b1 activated fibrosis in LX-2 cells was examined in the presence or absence of purified peptides NIPP-1 and NIPP-2. Besides the mechanisms of liver cell injury, protective effects of NIPP-1 and NIPP-2 were studied to show the protective mechanism against TGF-b1 stimulated fibrogenesis. Our results showed that the core protein of NIPP-1 peptide prevented fibril formation of type I collagen, elevated the MMP level and inhibited TIMP production in a dose-dependent manner. The treatment of NIPP-1 and NIPP-2 on TGF-b1 induced LX-2 cells alleviated hepatic fibrosis. Moreover, a-SMA, TIMPs, collagen and PDGF in the NIPP-1 treated groups were significantly decreased. Therefore, it could be suggested that NIPP-1 has potential to be used in anti-fibrosis treatment.
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KEYWORD
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microalgae, hepatic stellate cells, hepatic fibrosis, LX-2, liver injury
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