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KMID : 1160620140190040261
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Preventive Nutrition and Food Science
2014 Volume.19 No. 4 p.261 ~ p.267
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Anti-Inflammatory Activity of the Solvent-Partitioned Fractions from Spergularia marina in LPS-Stimulated RAW 264.7 Cells
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Kong Chang-suk
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Abstract
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As a part of ongoing research to elucidate and characterize antioxidant and anti-inflammatory nutraceu-ticals, solvent-partitioned fractions from Spergularia marina were tested for their ability to scavenge radicals and suppress inflammation. The results of the 2',7'-dichlorofluorescein diacetate assay indicate that solvent-partitioned fractions from S. marina scavenged intracellular radicals in H2O2-stimulated mouse macrophages. The tested fractions decreased the gen-eration of nitric oxide (NO) and the expression of inflammation mediators, namely, inducible nitric oxide synthase (iNOS) and interleukin (IL)-6, by lipopolysaccharide (LPS)-induced mouse macrophages, indicating that S. marina de-creases inflammation. Among all tested fractions [i.e., H2O, n-buthanol (n-BuOH), 85% aqueous methanol (aq. MeOH), and n-hexane], the 85% aq. MeOH fraction showed the strongest antioxidant and anti-inflammatory response. The 85% aq. MeOH fraction scavenged 80% of the free radicals produced by H2O2-induced control cells. In addition, NO pro-duction was 98% lower in 85% aq. MeOH fraction-treated cells compared to LPS-induced control cells. The mRNA ex-pression of iNOS and IL-6 was also suppressed in 85% aq. MeOH fraction-treated cells. The results of the current study suggest that the phenolic compound components of S. marina are responsible for its antioxidant and anti-inflammatory effects.
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KEYWORD
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antioxidant, anti-inflammatory, solvent fraction, halophyte, Spergularia marina
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