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KMID : 1160620140190040307
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Preventive Nutrition and Food Science
2014 Volume.19 No. 4 p.307 ~ p.313
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Structural Effects of Sulfated-Glycoproteins from Stichopus japonicus on the Nitric Oxide Secretion Ability of RAW 264.7 Cells
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Cao Rong-An
Lee Su-Han
You Sang-Guan
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Abstract
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The effect of various levels of proteins, sulfates, and molecular weight (Mw) of a sulfated-glycoprotein (NF3) from a sea cucumber, Stichopus japonicus, on nitric oxide (NO) releasing capacity from RAW 264.7 cells was investigated. The NF3 derivatives had various amounts of proteins (4.8∼11.2%) and sulfates (6.8∼25.2%) as well as different Mw (640.3×103∼109.2×103 g/mol). NF3 was able to stimulate RAW 264.7 cells to release NO with lower protein contents, indicating that the protein moiety was not an important factor to stimulate macrophages. On the other hand, the NO in-ducing capacity was significantly reduced with decreased levels of sulfates and Mw, implying that sulfates and Mw played a pivotal role in activating RAW 264.7 cells. It was not clear why sulfates and a certain range of Mw were essential for stim-ulating macrophages. It appeared that certain levels of sulfates and Mw of sulfated-glycoproteins were required to bind to the surface receptors on RAW 264.7 cells.
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KEYWORD
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Stichopus japonicus, sulfated-glycoproteins, structure-bioactivity, nitric oxide, RAW 264.7
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