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KMID : 1160620140190040353
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Preventive Nutrition and Food Science
2014 Volume.19 No. 4 p.353 ~ p.357
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Effects of Brown Rice Extract Treated with Lactobacillus sakei Wikim001 on Osteoblast Differentiation and Osteoclast Formation
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Kang Mi-Ran
Song Jung-Hee
Park Sung-Hee
Lee Jong-Hee
Park Hae-Woong
Kim Tae-Woon
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Abstract
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Phytic acid (myo-inositol hexakisphosphate) or phytate is considered an anti-nutrient due to the formation of precipitated complexes that strongly reduces the absorption of essential dietary minerals. In this study, brown rice with reduced phytate was made by inoculation with Lactobacillus sakei Wikim001 having high phytase activity. The effects of brown rice extract treated with L. sakei Wikim001 (BR-WK) on osteoblast differentiation and osteoclast formation were investigated. The proliferation of SaOS-2 cells was measured by the MTT assay. Treatment with BR-WK increased cell proliferation by 136% at a concentration of 100 μg/mL. The Alkaline phosphate activity in SaOS-2 cells was 129% higher when BR-WK was processed at a concentration of 100 μg/mL. The proliferation of bone marrow macrophages de-creased by nearly 60% in response to treatment with BR-WK. In addition, BR-WK reduced the number of tartrate-re-sistant acid phosphatase-positive (TRAP+) multinucleated cells from bone marrow macrophages. These results indicate that BR-WK stimulates bone formation through its positive action on osteoblast differentiation and function and fur-thermore, decreases osteoclast differentiation.
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KEYWORD
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brown rice, Lactobacillus sakei, phytate, osteoblast, osteoclast
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