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KMID : 1160620160210010038
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Preventive Nutrition and Food Science
2016 Volume.21 No. 1 p.38 ~ p.43
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Inhibition of MMP-2 and MMP-9 Activities by Limonium tetragonum Extract
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Bae Min-Joo
Karadeniz Fatih
Lee Seul-Gi
Seo Young-Wan
Kong Chang-suk
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Abstract
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Matrix metalloproteinases (MMPs) are crucial extracellular matrices degrading enzymes that take important roles in metastasis of cancer progression as well as other significant conditions such as oxidative stress and hepatic fibrosis. Natural products are on the rise for their potential to provide remarkable health benefits. In this context, hal-ophytes have been of interest in the nutraceutical field with reported instances of isolation of bioactive compounds. In this study, Limonium tetragonum, an edible halophyte, was studied for its ability to inhibit MMP-2 and -9 using HT1080 fi-brosarcoma cells. Results showed that L. tetragonum extract was able to inhibit the enzymatic activity and mRNA ex-pression of MMP-2 and -9 according to gelatin zymography and RT-PCR assays, respectively, but it was not able to sig-nificantly change the MMP pathway related factors such as tissue inhibitors of metalloproteinases. Also, Mitogen-acti-vated protein kinases pathway-related protein levels and their phosphorylation were assayed. While the phosphorylated p38 levels were decreased, extracellular signal-regulated kinase and c-Jun N-terminal kinase were not affected by L. tetra-gonum treatment. In conclusion, it was suggested that L. tetragonum contains substances acting as MMP inhibitors on en-zymatic activity rather than intracellular pathway intervention, which could be useful for further utilization of L. tetrago-num as a source for anti-MMP agents.
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KEYWORD
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HT1080, Limonium tetragonum, MMP, TIMP, MAPK
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