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KMID : 1160620160210010057
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Preventive Nutrition and Food Science
2016 Volume.21 No. 1 p.57 ~ p.61
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Changes of Mouse Gut Microbiota Diversity and Composition by Modulating Dietary Protein and Carbohydrate Contents: A Pilot Study
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Kim Eun-Jung
Kim Dan-Bi
Park Jae-Yong
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Abstract
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Dietary proteins influence colorectal cancer (CRC) risk, depending on their quantity and quality. Here, us-ing pyrosequencing, we compared the fecal microbiota composition in Balb/c mice fed either a normal protein/carbohy-drate diet (ND, 20% casein and 68% carbohydrate) or a high-protein/low-carbohydrate diet (HPLCD, 30% casein and 57% carbohydrate). The results showed that HPLCD feeding for 2 weeks reduced the diversity and altered the composi-tion of the microbiota compared with the ND mice, which included a decrease in the proportion of the family Lachno-spiraceae and Ruminococcaceae and increases in the proportions of the genus Bacteroides and Parabacteroides, especially the species EF09600_s and EF604598_s. Similar changes were reported in patients with inflammatory bowel disease, and in mouse models of CRC and colitis, respectively. This suggests that HPLCD may lead to a deleterious luminal environ-ment and may have adverse effects on the intestinal health of individuals consuming such a diet.
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KEYWORD
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gut microbiota, high protein diet, low carbohydrate diet, pyrosequencing, mouse
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