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KMID : 1160620160210030245
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Preventive Nutrition and Food Science
2016 Volume.21 No. 3 p.245 ~ p.254
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RAW264.7 Cell Activating Glucomannans Extracted from Rhizome of Polygonatum sibiricum
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Yelithao Khamphone
Surayot Utoomporn
Lee Ju-Hun
You Sang-Guan
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Abstract
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Water-soluble polysaccharides isolated from the rhizome of Polygonatum sibiricum and fractionated using ionexchange chromatography were investigated to determine their structure and immunostimulating activity. Crude and fractions (F1 and F2) consisted of carbohydrates (85.1∼88.3%) with proteins (4.51∼11.9%) and uronic acid (1.79∼7.47%), and included different levels of mannose (62.3∼76.3%), glucose (15.2∼20.3%), galactose (4.35∼15.3%), and arabinose (4.00∼7.65%). The crude contained two peaks with molecular weights (Mw) of 151×103 and 31.8×103, but F1 and F2 exhibited one major peak with Mw of 103×103 and 628×103, respectively. Little immunostimulatory activity was observed by the crude; however, F1 and F2 significantly activated RAW264.7 cells to release nitric oxide and various cytokines, suggesting they were potent immunostimulators. The backbone of the most immunostimulating fraction (F1) was (1→4)- manno- and (1→4)-gluco-pyranosyl residues with galactose and glucose attached to O-6 of manno-pyranoside.
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KEYWORD
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Polygonatum sibiricum, water-soluble polysaccharides, immunostimulation, molecular properties
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