|
KMID : 1160620160210040323
|
|
Preventive Nutrition and Food Science
2016 Volume.21 No. 4 p.323 ~ p.329
|
|
|
Achyranthes japonica Nakai Water Extract Suppresses Binding of IgE Antibody to Cell Surface FcεRI
|
|
Shim Sun-Yup
Lee Mi-Na
Lee Kyung-Dong
|
|
|
Abstract
|
|
|
|
Achyranthes japonica Nakai (AJN) water extract has a variety of physiological properties, including anti-diabetic, anti-cancer, anti-inflammatory, anti-microbial, and anti-oxidative activities. In the present study, the inhibitory effects of AJN extract were investigated in high affinity immunoglobulin E receptor (FcεRI)-mediated KU812F cells activation. AJN extract showed suppressive effects on histamine release and intracellular calcium [Ca2+]i elevation from anti- FcεRI antibody (CRA-1)-stimulated cells in a dose-dependent manner. Flow cytometric analysis showed that AJN extract treatment caused a dose-dependent decrease in the cell surface FcεRI expression and the binding between the cell surface FcεRI and the IgE antibody. Moreover, reverse transcription-polymerase chain reaction analysis showed that levels of the mRNA for the FcεRI α chain was decreased by treatment with AJN extract. These results indicate that AJN extract may exert anti-allergic effects via the inhibition of calcium influx and histamine release, which occurs as a result from the downregulation of the binding of IgE antibody to cell surface FcεRI. This mechanism may occur through FcεRI expression inhibition.
|
|
|
KEYWORD
|
|
|
Achyranthes japonica Nakai, histamine release, FcεRI, immunoglobulin E, human basophilic KU812F cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|