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KMID : 1160620180230040317
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Preventive Nutrition and Food Science
2018 Volume.23 No. 4 p.317 ~ p.325
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Inhibitory Effect of 20(S)-Ginsenoside Rg3 on Human Platelet Aggregation and Intracellular Ca2+ Levels via Cyclic Adenosine Monophosphate Dependent Manner
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Kwon Hyuk-Woo
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Abstract
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ABSTRACT: Intracellular Ca2+ ([Ca2+]i) induces platelet aggregation, and influences the activation of aggregation associated-molecules. The increased [Ca2+]i activates both the Ca2+/calmodulin-dependent phosphorylation of the myosin light chain and the diacylglycerol-dependent phosphorylation of pleckstrin to trigger granule secretion (i.e., dense body and α- granule) and platelet aggregation. This study was carried out to elucidate the antagonistic effect of 20(S)-ginsenoside Rg3 (G-Rg3) present in Panax ginseng Mayer on Ca2+. G-Rg3 inhibited thrombin-induced human platelet aggregation in a dose-dependent manner and suppressed thrombin-induced elevation of [Ca2+]i mobilization. G-Rg3 increased the levels of cAMP, and subsequently, elevated the phosphorylation of inositol 1,4,5-triphosphate receptor I (Ser1756) during thrombin-induced human platelet aggregation. Moreover, G-Rg3 inhibited thapsigargin-induced Ca2+ influx and the thrombin-induced elevation of extracellular signal-regulated kinase 2 phosphorylation. G-Rg3 exhibited an inhibitory effect on [Ca2+]i levels leading to granule release and thus a therapeutic potential against platelet-mediated thrombotic disease is suggested.
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KEYWORD
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20(S)-ginsenoside Rg3, inositol 1,4,5-triphosphate receptor I, extracellular signal-regulated kinases, p-selectin expression
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