KMID : 1161420170200070646
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Journal of Medicinal Food 2017 Volume.20 No. 7 p.646 ~ p.652
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3,3¡Ç-Diindolylmethane Suppresses Adipogenesis Using AMPK¥á-Dependent Mechanism in 3T3-L1 Adipocytes and Caenorhabditis elegans
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Lee Ji-Hye
Yue Yiren Park Yeon-Hwa Lee Seong-Ho
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Abstract
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3,3¡Ç-diindolylmethane is a major in vivo metabolite of indole-3-carbinol, a bioactive compound found in cruciferous vegetables. Although 3,3¡Ç-diindolylmethane has been implicated to possess antitumorigenic and anti-inflammatory properties, the effect of 3,3¡Ç-diindolylmethane on adipogenesis has not been explored previously. Thus, the present study was conducted to determine if 3,3¡Ç-diindolylmethane affects adipogenesis using 3T3-L1 adipocytes and Caenorhabditis elegans. Treatment of 3,3¡Ç-diindolylmethane significantly reduced fat accumulation without affecting viability in 3T3-L1 adipocytes. 3,3¡Ç-diindolylmethane suppressed expression of peroxisome proliferator-activated receptor ¥ã (PPAR¥ã), CCAAT-enhancer-binding protein ¥á (C/EBP¥á), fatty acid binding protein 4 (FABP4), and perilipin. In addition, 3,3¡Ç-diindolylmethane activated AMP-activated protein kinase ¥á (AMPK¥á), which subsequently inactivated acetyl CoA carboxylase (ACC), resulting in reduced fat accumulation. These observations were further confirmed in C. elegans as treatment with 3,3¡Ç-diindolylmethane significantly reduced body fat accumulation, which was partly associated with aak-1, but not aak-2, orthologs of AMPK¥á catalytic subunits ¥á1 and ¥á2, respectively. The current results demonstrate that 3,3¡Ç-diindolylmethane, a biologically active metabolite of indole-3-carbinol, may prevent adipogenesis through the AMPK¥á-dependent pathway.
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KEYWORD
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3,3¡Ç-diindolylmethane, adipogenesis, AMPK¥á, 3T3-L1, C. elegans
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