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KMID : 1161520000040030299
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Animal Cells and Systems
2000 Volume.4 No. 3 p.299 ~ p.304
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Stimulatory effects of extracellular products of mycobacterium spp. and various adjuvants on non-specific immune response of Nile Tilapia, oreochromis nilotica
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Choi Sang-Hoon
Oh Chan-Ho
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Abstract
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In the present paper, the immunostimulatory effects of the extracellular products (ECP) from Mycobacterium spp. and various adjuvants on the non?specific immune responses of Nile tilapia, Oreochromis nilotica, were examined. Nile tilapia were immunized by injecting ECP of Mycobacterium spp. (strain TB40, TB267 or the type strain Mycobacterium marinum) into their swim bladders. A variety of adjuvants like as Freund's complete adjuvant (FCA), Freund's incomplete adjuvant (FIA) and Titremax were similarly injected into additional groups of tilapia. The number of nitroblue tetrazolium (NBT)?positive cells observed in the swim bladder of the immunized fish was significantly increased by the fourth day post?immunization. By day 8, the numbers of NBT?positive cells were fewer in fish immunized with ECP from mycobacteria strains TB40 or TB267 than those immunized with ECP from M. marinum or fish injected with FCA or FIA. The level of lysozyme activity detected in the serum of fish 4 d after immunization with ECP from various Mycobacterium spp. was also significantly higher than that found in the serum of the control fish. Head kidney macrophages showed enhanced reduction of NBT when cultured in vitro with 1 pg/ml of ECP. Concentrations greater than this (10 or 100 pg/ml) were found to suppress the reduction of NBT by the macrophages. ECP from Mycobacterium spp. and the various adjuvants used in the study all appear to be good activators of the non?specific immune responses of Nile tilapia.
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KEYWORD
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ECP, Adjuvant, Nile tilapia, NBT, Mycobacteria, Oreochromis nilotica
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