KMID : 1161520110150020115
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Animal Cells and Systems 2011 Volume.15 No. 2 p.115 ~ p.121
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MK-801-induced learning impairments reversed by physostigmine and nicotine in zebrafish
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Choi Yong-Seok
Lee Chang-Joong Kim Yeon-Hwa
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Abstract
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Previous studies have demonstrated that N-methyl-D-aspartate (NMDA) receptors and acetylcholine receptors are related to learning and memory in rat and mice. In this study, we examined the effects of MK-801, a non-competitive NMDA receptor antagonist, on learning and memory in zebrafish using a passive avoidance test. We further tested whether or not nicotine, a nicotinic acetylcholine receptor agonist, and physostigmine, an acetylcholinesterase inhibitor, reverse the effects of MK-801. Crossing time was increased significantly in the training and test sessions for the controls. When 20 ¥ìM MK-801 was administered prior to the training session, the crossing time did not increase in either session. The MK-801-induced learning deficit was rescued by pretreatment with 20 ¥ìM physostigmine, and crossing time was increased in the training and test sessions compared to the MK-801-treated zebrafish. Further, the MK-801-induced learning deficit was prevented by pretreatment with 20 ¥ìM nicotine, and crossing time was increased in the training session but not in the test session. These results show that MK-801 induced a learning deficit in zebrafish that was prevented by pretreatment with nicotine and physostigmine.
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KEYWORD
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zebrafish, MK-801, physostigmine, nicotine, avoidance response
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