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KMID : 1161520120160040289
Animal Cells and Systems
2012 Volume.16 No. 4 p.289 ~ p.294
Identification of histone methyltransferase RE-IIBP target genes in leukemia cell line
Son Hye-Ju

Kim Ji-Young
Rhee Sang-Myung
Seo Sang-Beom
Abstract
Histone methylation has diverse functions including transcriptional regulation via its lysine or arginine residue methylation. Studies indicate that deregulation of histone methylation is linked to human cancers including leukemia. Histone H3K27 methyltrnasferase response element II binding protein (RE-IIBP), as a transcriptional repressor to target gene IL-5, interacts with HDAC and is over-expressed in leukemia patient samples. In this study, we have identified that hematopoiesis-related genes GATA1 and HOXA9 are down-regulated by RE-IIBP in K562 and 293T cells. Transient reporter analysis revealed that GATA1 transcription was repressed by RE-IIBP. On the other hand, HOXA9 and PBX-related homeobox gene MEIS1 was up-regulated by RE-IIBP. These results suggest that RE-IIBP might have a role in hematopoiesis or leukemogenesis by regulating the transcription of target genes, possibly via its H3K27 methyltransferase activity.
KEYWORD
RE-IIBP, histone methyltransferase, transcription, GATA1, MEIS1
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