KMID : 1161520180220010007
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Animal Cells and Systems 2018 Volume.22 No. 1 p.7 ~ p.14
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Phosphatidylcholine regulates NF-¥êB activation in attenuation of LPS-induced inflammation: evidence from in vitro study
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Chen Meijuan
Pan Hongying Dai Yining Zhang Jiajie Tong Yongxi Huang Yicheng Wang Mingshan Huang Haijun
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Abstract
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Phosphatidylcholine (PC) has been demonstrated as anti-inflammatory and antioxidant/pro-oxidant molecules. In this study, we investigated the protective effects of PC on inflammatory bowel disease (IBD) caused by lipopolysaccharide (LPS)-induced injury in intestinal epithelia cells. The IEC-6 cells (intestinal epithelia cells) were stimulated with LPS (1 ¥ìg/mL) for 24 h with or without PC pretreatment, in the next steps: (1) the level of the inflammatory cytokine tumor necrosis factor (TNF)-¥á was measured with ELISA; (2) the nuclear translocation and phosphorylation of NF-¥êB was investigated with Western blot, EMSA, immunofluorence assay; (3) the protein phosporylation levels in MAPK signaling pathway were detected with Western blot method. The results showed: (1) compared with the normal group, 10 and 20?¥ìg/mL of PC significantly inhibited the production and activation of TNF-¥á, (P???0.01); (2) pretreatment with PC inhibited LPS-induced nuclear translocation and phosphorylation of p65 in IEC-6 cells; (3) pretreatment with PC inhibited the protein phosphorylation levels in MAPK signaling pathway. Our findings indicated that PC had the effect to protect IEC-6 cells from LPS-induced injury and this effect was exerted possibly through inhibiting the TNF-? secretion, down-regulating nuclear translocation and phosphorylation of p65 and inhibiting MAPK signaling pathways.
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KEYWORD
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Phosphatidylcholine, LPS-induced injury, tTNF-¥á secretion and activation, p65 nuclear translocation, MAPKs signal pathway
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