KMID : 1195620140070010001
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Clinical and Experimental Otorhinolaryngology 2014 Volume.7 No. 1 p.1 ~ p.6
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Expression Profile of Fas-Fas Ligand in Spiral Ganglion Cells During Apoptosis
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Gu Tae-Woo
Bae Woo-Yong Park Hwan-Tae Lee Jae-Hoon Kang Min-Young Jeong Sung-Wook Shin Yoon-Kyung
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Abstract
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Objectives: To examine the expression profile of Fas-Fas ligand (FasL) during glutamate (Glu)-induced spiral ganglion cell (SGC) apoptosis.
Methods: Cultured SGCs were treated with 10-mM, 25-mM, and 50-mM concentrations of Glu and incubated for 24 or 48 hours. The expression intensity of FasL, Fas, caspase 3, and morphology of single SGC were evaluated using immunofluorescence staining.
Results: In semiquantitative analysis of the Glu-treated SGC, FasL, and caspase 3 expression intensity were increased with concentration- and time-dependent manner. Fas expression intensity did not change with different concentration at 48 hours. In morphologic analysis of the Glu-treated SGC, number of apoptotic cells were increased with concentration- and time-dependent manner.
Conclusion: FasL was expressed in apoptotic SGCs, suggesting that the Fas-FasL signaling pathway may be involved in the Glu-induced apoptosis of dissociated SGCs.
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KEYWORD
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Spiral ganglion cell, Glutamate, Apoptosis, Fas ligand, Caspase 3
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