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KMID : 1200820000010020045
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Oriental Pharmacy and Experimental Medicine
2000 Volume.1 No. 2 p.45 ~ p.51
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Cytotoxicity and antimicrobial effects of the methanolic extract of Sophora flavescens Ait. (IV)
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Baek Seung-Hwa
Kang Kil-Ung
Lee Jeong-Ho
Park Nang-Kyu
Chai Kyu-Yun
You Il-Soo
Kim Jong-Soo
Ryu Do-Gon
Lee Kang-Min
Yang Eun-Yeong
Lee Hyun-Ok
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Abstract
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This study was carried out to evaluate cytotoxicity of the methanol extract from Sophora flavescens Ait. against L1210 (lymphocytic leukemia) and (lymphoid neoplasma) Cells in vitro. We have determined cytotoxicity by the MTT (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H- tetrazolium bromide) assay. The order of cytotoxicity of Sophora flavescens Ait. extracts against L1210 and cells in vitro is as follows: Fr. 4 > Fr. 3 > Fr. 5 > Fr. 2 > Fr. 1. These results suggest that the fraction 4 of the methanol extracts from Sophora flavescens Ait. may be a valuable choice for the development of antitumor agents. In order to develop an antimicrobial agent, dried Sophora flavescens Ait. was extracted with hot methanol, and then antimicrobial activity (MIC test) was investigated. In this study, the fraction 3 of the methanol extracts from the roots of S. flavescens showed strong growth inhibition activity against gram-positive and gram-negative bacteria (MIC, ) such as S. mutans, S. epidermidis and P. putida. These results indicate that fractions 3 and 4 inhibit tumor cells and bacteria.
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KEYWORD
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Antimicrobial agent, Sophora flavescens Ait, Minimum inhibitory concentration (MIC), Cytotoxicity
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