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KMID : 1200820000010020059
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Oriental Pharmacy and Experimental Medicine
2000 Volume.1 No. 2 p.59 ~ p.65
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Effect of Rhizoma gastrodiae on oxidative stress in cultured mouse spinal motor neurons
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Park Seung-Taeck
Kim Jeong-Joong
Jeong Se-Jin
Cho Chung-Gu
Lee Kang-Chang
Jang Chul-Ho
Park Jae-Hwang
Hong Kee-Yeon
Yoon Hyang-Suk
Oh Yeon-Kyun
Oh Kwang-Su
Min Bu-Kie
Han Du-Seok
Baek Seung-Hwa
Chun Seung-Ho
Lee Gap-Sang
Lee Seong-Keun
Sung Kang-Keyng
Lee Geon-Mok
Jeon Byung-Hoon
Song Ho-Jun
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Abstract
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To clarify the toxic effect of oxidative stress, hydrogen peroxide neurotoxicity was examined in cultured newborn mouse spinal motor neurons after spinal motor neurons were grown in the media containing various concentrations of glucose oxidase (GO). And also, the protective effect of Rhizoma gastrodiae extract against GO-induced neurotoxicity was evaluated. Cytotoxicity was expressed as a cell viability by 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay. In this study, exposure of motor neurons to GO-induced cell death significantly, in a dose- and time-dependent manners in spinal motor neuron cultures. The decrease in cell viability of motor neurons damaged by GO was proventioned by Rhizoma gastrodiae extract. These results suggest that the neuroprotective effect of Rhizoma gastrodiae extract on GO-induced neurotoxicity may result from a attenuation of oxidative stress.
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KEYWORD
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Rhizoma gastrodiae extract, Glucose oxidase, Oxidative stress
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