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KMID : 1200820030030020100
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Oriental Pharmacy and Experimental Medicine
2003 Volume.3 No. 2 p.100 ~ p.105
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Multidrug resistance reversal in mouse lymphoma cells by indian tea leaves, indian coffee seeds and chicory
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Rao Bhattiproulu Kesava
Motohashi Noboru
Spengler Gabriella
Molnar Joseph
Kawase Masami
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Abstract
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Systematic analysis of caffeine from the commercial samples of Indian tea leaves was performed by a routine method and the content of caffeine was found to be 19.0-37.4 mg/100 g leaves. The caffeine contents from coffee seeds and chicory from Indian origin were analyzed and found to be 0.6540-1.4920 g/100 g seeds. Caffeine contents of roasted Indian chicory roots were lower than either those of Indian tea leaves or Indian coffee seeds. The multidrug resistance (MDR) reversing effects were tested on a mouse leukemia cell line of L-5178 cells by methanol extracts [M1-M15] of Indian tea leaves and coffee seeds, comparing to a control of . The effects were measured by fluorescence ratio between treated and untreated group cells. Among fifteen methanol extracts, a Gemini tea [M6] (fluorescence activity ratio 5.26) had the most potent effect for L-5178 cells. The extract M6 was 0.63-fold of . We suggest that one of mechanisms of reversal by M6 might have strong affinity to dopamine and receptors. Further studies with many more tumor and normal cell lines are necessary to confirm the MDR reversal specificity of coffee methanol extracts.
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KEYWORD
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Indian teas, Caffeine, Chicory, Methanol extracts, MDR reversal
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