Àá½Ã¸¸ ±â´Ù·Á ÁÖ¼¼¿ä. ·ÎµùÁßÀÔ´Ï´Ù.
KMID : 1200820030030030151
Oriental Pharmacy and Experimental Medicine
2003 Volume.3 No. 3 p.151 ~ p.156
Induction of apoptosis by protein fraction isolated from the leaves of Mirabilis jalapa L on HeLa and Raji cell-line
Ikawati Zullies

Sudjadi
Elly Widyaningsih
Puspitasari Dyah
Sismindari
Abstract
The leaves of Mirabilis jalapa L contains protein fraction presumed ribosome-inactivating protein (RIP). RIP is a group of protein that has RNA N-glycosidase activity that is capable to inhibit protein synthesis. Protein fraction of the plant was shown to be cytotoxic on HeLa cell-line, however, the mechanism by which the protein kill the cells is not identified yet, whether trough apoptosis, necrosis, or other mechanism. This research aim to study the mechanism of cell death caused by the protein fraction isolated from the leaves of this plant on HeLa and Raji cell-line, as representative of different kind of cancer cells. Results showed that protein fraction isolated from the leaves of Mirabilis jalapa L was more cytotoxic to HeLa cell-line (LC50: 0.65 mg/ml) than to Raji cell-line (1.815 mg/ml) on 48 hours incubation time. Moreover, it was demonstrated that the death of HeLa cells caused by the protein fraction was due to induction of apoptosis, while on Raji cell-line was due to non-apoptosis way, presumably via necrosis.
KEYWORD
Ribosome-inactivating protein (RIP), Leaves of Mirabilis jalapa L, Cytotoxicity, Apoptosis, HeLa and Raji cell-line
FullTexts / Linksout information
Listed journal information
ÇмúÁøÈïÀç´Ü(KCI)