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KMID : 1200820040040040227
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Oriental Pharmacy and Experimental Medicine
2004 Volume.4 No. 4 p.227 ~ p.234
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Purification and partial characterization of from soybean (Glycine max)
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Tripathi Pallavi
Dwevedi Alka
Kayastha Arvind M.
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Abstract
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An was purified to apparent homogeneity from germinating soybean seeds (Glycine max). Enzyme showed high specificity for starch. from soybean has optimum pH at 7.6 in the pH range 4.0-10.6. At this pH, the of starch was 2.63 mg/ml and the was equal to 52.6 mg/ml/min protein. Optimum temperature of the enzyme was found to be equal to 1.85 and energy of activation equal to 12 kcal/mol. Additives like, EDTA reduced the activity of whereas PMSF enhanced the activity. was inhibited by several heavy metal ions.
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KEYWORD
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Purification, Starch, Soybean, Substrate specificity, Inhibitors
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