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KMID : 1200820070070040341
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Oriental Pharmacy and Experimental Medicine
2007 Volume.7 No. 4 p.341 ~ p.347
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Ethanol extract of Callophyllis japonica enhances nitric oxide and tumor necrosis factor-alpha production in mouse macrophage cell line, RAW 264.7 cells
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Ahn Mee-Jung
Park Dal-Soo
Yang Won-Hyung
Go Gyung-Min
Kim Hyung-Min
Hyun Jin-Won
Park Jae-Woo
Shin Taek-Yun
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Abstract
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Red seaweed (Callophyllis japonica) has long formed part of the diet of Asians, but the pharmacological properties of this plant have not been evaluated. In this study, we examined the effect of an ethanol extract of C. japonica on the generation of nitric oxide (NO) in RAW 264.7 cells. The C. japonica extract increased the generation of NO and tumor necrosis (), which were detected by the Griess method and an enzyme-linked immunosorbent assay, respectively. The increased production of NO by C. japonica extract was inhibited by -monomethyl-L-arginine (), a specific inhibitor of NO production in the L-arginine-dependent pathway, and by the nuclear () inhibitor, pyrrolidine dithiocarbamate () in a dose-dependent manner. These findings demonstrate that C. japonica extract stimulates the production of NO and in RAW 264.7 cells through the activation of and that this extract might also inhibit the growth of the human leukemic cells.
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KEYWORD
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Callophyllis japonica, Red seaweed, Nitric oxide, RAW 264.7 cells, Tumor necrosis factor-
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