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KMID : 1200820080070050549
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Oriental Pharmacy and Experimental Medicine
2008 Volume.7 No. 5 p.549 ~ p.555
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Modulation of the aqueous extract of Bupleuri radix on glycine-induced current in the acutely dissociated rat periaqueductal gray neurons
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Sung Yun-Hee
Shin Mal-Soon
Kim Tae-Soo
Lee Sang-Won
Kim Youn-Jung
Shin Hye-Sook
Kim Chang-Ju
Kim Hong
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Abstract
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Bupleuri radix (Umbelliferae), the dried root of Bupleurum Chinense DC, has been clinically used to mitigate pain sensation. The descending pain control system consists of three major components, and modulation of pain in the periaqueductal gray is the most extensively studied descending pain control system. However, the relation of Bupleuri radix on the descending pain control system has not been clarified. In the present study, modulation of the aqueous extract of Bupleuri radix on glycine-induced ion current in the acutely dissociated periaqueductal gray neurons was investigated by using nystatin-perforated patch-clamp technique under voltage-clamp condition. In the present results, the glycine-induced ion current was significantly suppressed by 0.1 mg/ml Bupleuri radix, while treatment with naltrexone, opioid receptor antagonist, alleviated Bupleuri radix-induced inhibition on glycine-induced ion current. The present study showed that the aqueous extract of Bupleuri radix may activate descending pain control system through inhibition on glycine-induced ion current in the periaqueductal gray neurons and this effect is mediated by opioid receptors.
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KEYWORD
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Bupleuri radix, Glycine, Periaqueductal gray, Naltrexone, Patch Clamp
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