KMID : 1200820080080030222
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Oriental Pharmacy and Experimental Medicine 2008 Volume.8 No. 3 p.222 ~ p.227
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Effect of Citrus aurantium var amara on weight change in mice
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Sarker Satyajit Dey
Habibi Bohlol Sharifi Tohid Asnaashari Solmaz Nahar Lutfun Delazar AndAbbas
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Abstract
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Citrus aurantium var. amara L., commonly known as ¡¯bitter orange¡¯ or ¡¯sour orange¡¯, of the family Rutaceae, has traditionally been used in the treatment of various ailments, and it possesses different types of pharmacological properties. As a part of our on-going studies on the plantsfrom the Iranian flora, the extract of C. aurantium var. amara has been studied for its weight lossproperties using the mice model. While the Sep-Pak fraction, 20% methanol (MeOH) in water, of the hydro-methanolic extract of the peels of C. aurantium var. amara fruits, when injectedintraperitoneal (i.p.) at a dose of 10 mg/kg, significantly decreased the level of weight gain of the mice in comparison with control the group (P < 0.01), the Sep-Pak fraction 80% MeOH in water decreased the initial weight of mice by 0.44% in six weeks. The administration of the total extract(10 and 20 mg/kg, i.p.), and the Sep-Pak fractions, 40% and 60% MeOH in water (10 mg/kg, i.p.)did not show any significant change of weight of the test mice. Of the two active fractions, the80% MeOH in water fraction did not show any noticeable adverse effects on mice, and was therefore analysed by reversed-phase preparative high performance liquid chromatography resulting in the isolation and identification of four major components, two coumarins, meranzin hydrate (1) and bergamottin (2), and two flavonoids, xanthomicrol 5,4¡¯-di-methyl ether (tangeritin, 3) and hymenoxin 5,7-di-methyl ether (nobiletin, 4).
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KEYWORD
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Rutaceae, Citrus aurantium var. amara, Coumarin, Flavonoid, Weight reduction
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