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KMID : 1200820100100010007
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Oriental Pharmacy and Experimental Medicine
2010 Volume.10 No. 1 p.7 ~ p.12
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Typha orientalis inhibits inflammatory cytokine expression through suppression of ERK phosphorylation in HMC-1 cells
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Choi In-Young
Na Ho-Jeong
Um Jae-Young
Kim Hyung-Min
Hong Seung-Heon
Sim Kuk-Jin
Song Bong-Keun
Nam Gi-Hye
Choung Se-Young
Jeong Hyun-Ja
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Abstract
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Typha orientalis’ stem (TOS) is traditionally used as an herbal medicine for difficulty in urination, galactophoritis purulenta, whooping cough, and allergic dermatitis. However, its effect in experimental models remains unknown. Here, we report the effect of TOS on the phorbol 12-myristate 13- acetate (PMA) plus calcium ionophore A23187-induced inflammatory cytokine production and extracellular signal-regulated kinase (ERK) activation in the human mast cell line, HMC-1. TOS
inhibited PMA plus A23187-induced cytokines such as tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6. Maximal inhibition rate of TNF-α and IL-6 production by TOS (1 mg/ml) was about 44.02%, and 45.20%, respectively (P < 0.05). In addition, TOS inhibited the expression of TNF-α and IL-6 mRNA under the same condition. Moreover, TOS partially blocked PMA plus A23187-induced ERK phosphorylation. These results suggested TOS could inhibit the cytokine
production through blocking of ERK activity.
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KEYWORD
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Human mast cell, Typha orientalis’ stem, Phorbol 12-myristate 13-acetate, A23187, Tumor necrosis factor-, Interleukin-6, Extracellular signal-regulated kinase
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