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KMID : 1200820100100030155
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Oriental Pharmacy and Experimental Medicine
2010 Volume.10 No. 3 p.155 ~ p.164
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Aqueous extract of Lycii fructus suppresses inflammation through the inhibition of nuclear factor kappa B signal pathway in murine raw 264.7 macrophages
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Kim Beum-Seuk
Lim Hyung-Ho
Song Yun-Kyung
Sung Yun-Hee
Kim Sung-Eun
Chang Hyun-Kyung
Shin Mal-Soon
Kim Chang-Ju
Lee Hye-Jung
Kim Dong-Hee
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Abstract
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Lycii fructus is the fruit of Lycium chinense Miller and is part of the Solanaceae family. Lycii fructus produces various effects such as hypotensive, hypoglycemic, anti-pyretic, and anti-stress activities. Lycii fructus is known to contain betaine, carotene, nicotinic acid, zeaxanthin, and cerebroside. In the present study, the effects of Lycii fructus aqueous extract on lipopolysaccharide (LPS)-induced inflammation in murine raw 264.7 macrophage cells were investigated. In this study we utilized the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, reverse transcriptionpolymerase chain reaction (RT-PCR), Western blotting, and nitric oxide (NO) detection. Lycii fructus aqueous extract suppressed NO production by inhibiting the LPS-induced expressions of inducible nitric oxide synthase (iNOS) and tumor necrosis factor-alpha (TNF-) mRNA and iNOS protein in murine raw 264.7 macrophage cells. Also, Lycii fructus aqueous extract suppressed the activation of nuclear factor-kappa B (NF-) in the nucleus. These results demonstrated that Lycii fructus aqueous extract causes an anti-inflammatory effect that was likely produced by the suppression of iNOS expression through the down-regulation of NF- binding activity.
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KEYWORD
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Lycii fructus, Lipopolysaccharide, Inducible nitric oxide synthase, Nitric oxide, Nuclear factor-kappa B
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