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KMID : 1200820120120040273
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Oriental Pharmacy and Experimental Medicine
2012 Volume.12 No. 4 p.273 ~ p.277
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ACE inhibiton activity of standardized extract and fractions of Terminalia bellerica
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Chaudhary Sushil Kumar
Mukherjee Pulok K.
Nema Neelesh Kumar
Bhadra Santanu
Saha B. P.
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Abstract
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The fruit of Terminalia bellerica L. (Combretaceae) is an important ingredient of ‘Triphala’, which is a popular Ayurvedic formulation traditionally used to treat hypertension, to reduce cardiac depression and to decreases the risk factors associated with the heart. This study aimed to investigate the angiotensin converting enzyme (ACE) inhibitory activity of T. bellerica. Standardized hydro alcoholic extract (TBHA) and its various subfractions including hexane fraction (TBH), ethyl acetate fraction (TBE), n-butanol fraction (TBB) and aqueous fraction (TBW) at the concentration of 10?1000 μg/ml together with standard Captopril 3.6 ng/ml was compared. TBE fraction was undertaken to isolate the gallic acid. Further quantification of gallic acid in the crude extract and fractions was made with HPLC. Among all fractions the activity was found to be maximum in TBE with an IC50?=?338.54?±?18.34 μg/ml while crude TBHA and other fractions TBB, TBH and TBW were found less potent. Isolated gallic acid from the TBE fraction shown ACE inhibitory activity with IC50 of 257.29?±?9.39 μg/ml. TBE found to contain maximum amount of gallic acid (71.05?±?6.274 mg/g of extract). The presence of gallic acid along with other metabolites in the extract and fractions might be responsible for the ACE inhibitory activity. T. bellerica extract/fractions suggested its suitability as a functional food for pharmaceutical purpose to be used against hypertension and other related diseases.
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KEYWORD
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ACE inhibitor, Gallic acid, Terminalia bellerica, Hypertension, HPLC
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