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KMID : 1200820130130030205
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Oriental Pharmacy and Experimental Medicine
2013 Volume.13 No. 3 p.205 ~ p.216
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Anti-tumor activity of Swietenia mahagoni (L.) Jacq. leaf extract against Ehrlich’s ascites carcinoma in mice
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Roy Soma
Banerjee Bisweswar
Vedasiromoni Joseph R.
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Abstract
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The anti-proliferative, cytotoxic and apoptotic activities of Swietenia mahagoni (L.) Jacq. leaf extract (SMLE) was investigated against Ehrlich’s ascites carcinoma (EAC) in mice. SMLE significantly inhibited the growth and the metabolic activity of viable EAC cells in a dose- and time-dependent manner as observed in both in vivo and in vitro studies and also showed characteristic features of apoptosis. Flow cytometric analysis showed appreciable number of cells in early and late apoptotic stages. Cell cycle analysis revealed that treatment with SMLE arrested the EAC cell populations in the G2-M phase of cell cycle. SMLE induced apoptosis is mediated through mitochondrial intrinsic pathway involving the release of cytochrome c into the cytosol and activation of caspase-9 and caspase-3. The two flavonoids, catechin and quercetin-3-O-glucoside isolated from Swietenia mahagoni (L.) Jacq. leaves, which have been found to be the active ingredients responsible for the anti-leukemic activity of SMLE, may be responsible for the activity of SMLE on EAC.
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KEYWORD
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Swietenia mahagoni (L.) Jacq. leaf extract, Antiproliferative activity, Cytotoxic activity, Apoptotic activity, Mitochondria mediated intrinsic pathway
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