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KMID : 1200820140140010015
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Oriental Pharmacy and Experimental Medicine
2014 Volume.14 No. 1 p.15 ~ p.24
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A critical biochemical assessment on the antihyperglycemic activity of aqueous fraction of Wattakaka volubilis supported by antioxidant defense
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Gopal Velmani
Mandal Vivekananda
Mandal Subhash C.
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Abstract
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To evaluate DPPH, nitric oxide, hydrogen peroxide, reducing power assay and in vivo antihyperglycemic activity of aqueous fraction of Wattakaka volubilis against streptozotocin induced diabetes in male Wistar albino rats. The antioxidant potential of aqueous fraction was exhibited with 50?250 μg/mL concentration. Hyperglycemia was induced in rats by single intraperitoneal injection of streptozotocin (60 mg/kg, bw). 48 h after STZ induction, the hyperglycemic rats were treated with aqueous fraction of Wattakaka volubilis orally at the single dose of 200 mg/kg, bw daily for 21 days. Metformin (500 mg/kg bw, orally) was used as standard drug. The fasting blood-glucose levels were measured on 0th, 7th, 14th and 21st days during the treatment regimen. Serum biochemical parameters such as glucose, sodium, potassium, creatinine, urea, alanine aminotransferase (ALT), alkaline phosphatase (ALP) was studied on untreated and treated groups. Oral glucose tolerance test and glycogen content of liver, skeletal muscle was estimated. Histological examinations on liver, pancreas and kidney section were analyzed to assess the impact of the aqueous fraction. Treatment of aqueous fraction in experimental rats by oral injections for 21 days showed reductions in the levels of serum biochemical markers. Histopathological studies of tissue samples further ascertained the protective effects of aqueous fraction in diabetic rats. The present study indicates that active principle(s) in the aqueous fraction responsible for normalizing the biochemical changes in diabetic rats.
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KEYWORD
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Wattakaka volubilis, DPPHNitric oxide, Antihyperglycemia, Streptozotocin, Pancreas
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